Application
Western blot analysis of protein 4.2 expression in TF-1 (A), human erythocyte (B), Hep G2 (C) and human platelet (D) whole cell lysates.| Product Name | protein 4.2 Antibody (M-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against protein 4.2 of human origin |
| Modification Notes | Protein 4.2, also known as Erythrocyte membrane protein band 4.2, is a 691 amino acid transmembrane protein, which possibly regulates mechanical and morphological properties of erythrocytes. Protein 4.2 functions to strongly influence CD47 levels and also regulate the association between Ankyrin and protein 3. Appearing on erythroblasts at a very late stage of red blood cell development, protein 4.2 is predominantly found in liver and is also expressed in spleen, stomach, testis and eye. One of several members of the transglutaminase family, protein 4.2 is subject to a loss of function caused by an amino acid substitution from Cys to Ala in the active site. Complete or partial protein 4.2 absence leads to a weaker than usual association of ankyrin to the membrane skeleton. Defects in the gene encoding protein 4.2 are the cause of hereditary spherocytosis, a hematologic disorder characterized by abnormally shaped erythrocytes and chronic hemolytic anemia. A short and long isoform of protein 4.2 exist as a result of an alternative splicing event. The short isoform is characterized as the major protein 4.2 species in human erythrocyte membranes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of protein 4.2 of h and r origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against protein 4.2 of human origin |
|---|---|
| Tissue Specificity | raised against protein 4.2 of human origin |
| Entrez Gene | 2038 |
|---|
Application
Western blot analysis of protein 4.2 expression in TF-1 (A), human erythocyte (B), Hep G2 (C) and human platelet (D) whole cell lysates.| Application Notes | recommended for detection of protein 4.2 of h and r origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
