Application
Western blot analysis of Ubr7 expression in non-transfected: (A) and human Ubr7 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Urb7 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining.| Product Name | Ubr7 Antibody (G-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against Ubr7 of human origin |
| Modification Notes | Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). Ubr7 (ubiquitin protein ligase E3 component n-recognin 7), also known as C14orf130 or N-recognin-7, is a 425 amino acid protein that contains one UBR-type zinc finger and one PHD zinc finger. Participating in protein modification events within the N-end rule pathway, Ubr7 functions as an E3 ubiquitin-protein ligase that recognizes and binds proteins that contain destabilizing N-terminal residues, thereby leading to their ubiquitination and subsequent degradation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ubr7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against Ubr7 of human origin |
|---|---|
| Tissue Specificity | raised against Ubr7 of human origin |
| Entrez Gene | 55148 |
|---|
Application
Western blot analysis of Ubr7 expression in non-transfected: (A) and human Ubr7 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Urb7 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining.| Application Notes | recommended for detection of Ubr7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
