Application
Antibodies tested include p-paxillin (Tyr 118): (A, B) and paxillin (H-114): (C, D).Application
Western blot analysis of paxillin phosphorylation in untreated (A), pervanadate treated (B) and pervanadate and lambda protein phosphatase (A) treated (C) A-431 whole cell lysates.Application
Western blot analysis of phosphorylated paxillin expression in untreated (A) and EGF-treated (B) HeLa whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-paxillin Antibody (Tyr 118) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 118 of paxillin of human origin |
| Modification | Phosphoralated |
| Modification Notes | The effects of some oncogenes, growth factors and neuropeptides are mediated by tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin cytoskeletal proteins. A rapid increase in tyrosine phosphorylation of paxillin, FAK and Crk-associated substrate (CAS) are prominent early events triggered by many G-protein-coupled receptors. In addition to G protein-coupled receptors, angiotensin IV (Ang IV), protein kinase C and other proteins can also mediate the tyrosine phosphorylation of paxillin. Paxillin must bind FAK for maximal phosphorylation in response to cell adhesion. FAK may function to direct tyrosine phosphorylation of paxillin in the process of transformation by the src oncogene. Tyrosine phosphorylated FAK and paxillin function to regulate the signaling mechanism of the rapid nongenomic action of dexamethasone on the Actin cytoskeleton. In glomerular epithelial cells, TNFa induces substantial reorganization of Actin cytoskeleton and focal adhesions. TNFa also simultaneoulsy mediates tyrosine phosphorylation of paxillin and FAK, which regulate Actin polymerization and the formation of focal adhesions, and may be directly involved in the redistribution of Actin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Tyr 118 phosphorylated paxillin of human and mouse origin and correspondingly phosphorylated Tyr 140 of rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 118 of paxillin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 118 of paxillin of human origin |
| Entrez Gene | 5829 |
|---|
Application
Antibodies tested include p-paxillin (Tyr 118): (A, B) and paxillin (H-114): (C, D).
Application
Western blot analysis of paxillin phosphorylation in untreated (A), pervanadate treated (B) and pervanadate and lambda protein phosphatase (A) treated (C) A-431 whole cell lysates.
Application
Western blot analysis of phosphorylated paxillin expression in untreated (A) and EGF-treated (B) HeLa whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Tyr 118 phosphorylated paxillin of human and mouse origin and correspondingly phosphorylated Tyr 140 of rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
