Application
Western blot analysis of KLHL13 expression in non-transfected: (A) and human KLHL13 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of KLHL13 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human intestine tissue showing cytoplasmic staining.| Product Name | KLHL13 Antibody (O-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against KLHL13 of human origin |
| Modification Notes | KLHL13 (kelch-like 13), also known as BKLHD2 (BTB and kelch domain-containing protein 2), is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain. Expressed predominantly in brain, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation. Specifically, KLHL13 interacts with KLHL9 and CUL-3, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin-mediated proteolysis. Via its BTB and C-terminal Kelch (BACK) motif, KLHL13 is thought to play a role in spatially orientating substrates in the CUL-3 ligase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of KLHL13 of human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against KLHL13 of human origin |
|---|---|
| Tissue Specificity | raised against KLHL13 of human origin |
| Entrez Gene | 90293 |
|---|
Application
Western blot analysis of KLHL13 expression in non-transfected: (A) and human KLHL13 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of KLHL13 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human intestine tissue showing cytoplasmic staining.| Application Notes | recommended for detection of KLHL13 of human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
