Application
Western blot analysis of Elf-4 expression in non-transfected: (A) and human Elf-4 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).| Product Name | Elf-4 Antibody (M-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against Elf-4 of human origin |
| Modification Notes | The Ets-1 family of transcription factors has a conserved DNA binding domain through which it plays an important role in cellular proliferation, differentiation, tematopoiesis and angiogenesis. This domain, also known as the Ets domain, binds to DNA sequences containing the consensus sequence 5'-WGGA-3’, which is known as the Ets-binding domain. Elf-4, also known as myeloid Elf-1-like factor, ELF4 or MEF, is a 663 amino acid member of the Ets-1 family. Localized to the nucleus, Elf-4 is highly expressed in placenta and myeloid leukemia cells, with lower levels of expression lung, heart, thymus, slpeen, colon, ovary and peripheral blood lymphocytes. Functioning primarily to activate the promoters of hematopoietic growth factor genes, such as GM-CSF, IL-3 and IL-8, Elf-4 has also been shown to activate the Perforin 1 promoter in natural killer (NK) cells, suggesting a possible role in tumorigenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| recommended for detection of Elf-4 of human origin by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against Elf-4 of human origin |
|---|---|
| Tissue Specificity | raised against Elf-4 of human origin |
| Entrez Gene | 2000 |
|---|
Application
Western blot analysis of Elf-4 expression in non-transfected: (A) and human Elf-4 transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).| Application Notes | recommended for detection of Elf-4 of human origin by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
