Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of CPTI-M expression in mouse heart (A) and rat skeletal muscle (B) tissue extracts.| Product Name | CPTI-M Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 16-135 mapping near the N-terminus of CPTI-M, muscle isoform, of human origin |
| Modification Notes | The mitochondrial ∫-oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase (CPT) I (outer membrane and detergent labile) and II (inner membrane and detergent stable), together with carnitine carrier. CPTI catalyzes the first reaction in the transport of long-chain fatty acids from the cytoplasm to the mitochondrion, a rate-limiting step in beta-oxidation. Two types of CPTI are known, the liver (CPTIA) and muscle (CPTIB) isoforms. The muscle type protein is specially expressed in heart and skeletal muscle. Membrane-bound CPTI, but not CPTII, is inhibited reversibly by malonyl-coenzyme A (CoA). Unlike CPTII, CPTI requires membrane integrity for catalytic function. In addition, glutamic acid 3 and histidine 5 are necessary for malonyl CoA inhibition and binding to liver CPTI, but not for catalytic activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CPTI-M of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 16-135 mapping near the N-terminus of CPTI-M muscle isoform of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 16-135 mapping near the N-terminus of CPTI-M, muscle isoform, of human origin |
| Entrez Gene | 1375 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of CPTI-M expression in mouse heart (A) and rat skeletal muscle (B) tissue extracts.| Application Notes | recommended for detection of CPTI-M of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
