Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Furin expression in non-transfected: (A) and mouse Furin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Furin expression in mouse liver tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Furin expression in HeLa (A), MDCK (B), Hep G2 (C), C6 (D) and c4 (E) whole cell lysates and mouse liver tissue extract (F).| Product Name | Furin Antibody (H-220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 575-794 mapping at the C-terminus of Furin of human origin |
| Modification Notes | Furin (FUR, PACE, PCSK3, SPC1, Kex2p) is a calcium-dependent serine endoprotease that belongs to the subtilisin-like proprotein convertase family. The members of this family process latent precursor proteins into their biologically active products. Furin cleaves at paired basic amino acid processing sites within proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. Furin can directly cleave proMMP-2 within the trans-Golgi network leading to an inactive form of matrix metalloproteinase-2 (MMP-2). Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The inhibitory mechanism is based on the presence of an inactivating prosegment at the NH2 terminal of the Furin. After initial autocatalytic cleavage, the prosegment remains tightly associated until it reaches the trans-Golgi network where the dissociation of the prosegment and activation of furin occurs. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Furin of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 575-794 mapping at the C-terminus of Furin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 575-794 mapping at the C-terminus of Furin of human origin |
| Entrez Gene | 5045 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Furin expression in non-transfected: (A) and mouse Furin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Furin expression in mouse liver tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Furin expression in HeLa (A), MDCK (B), Hep G2 (C), C6 (D) and c4 (E) whole cell lysates and mouse liver tissue extract (F).| Application Notes | recommended for detection of Furin of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
