Application
Western blot analysis of PLC γ2 expression in non-transfected 293T: (A), human PLC γ2 transfected 293T: (B) and MCF7 (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse lymph node frozen section showing membrane and cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing membrane and cytoplasmic localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lymphoma showing membrane and cytoplasmic staining.Application
Western blot analysis of PLC γ2 expression in MCF7 (A), MCF7 + etoposide (B), U-937 (C) and THP-1 (D) whole cell lysates.| Product Name | PLC γ2 Antibody (Q-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of PLC γ2 of human origin |
| Modification Notes | Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.There are many mammalian PLC isozymes, including PLC ∫1, PLC ∫2, PLC ∫3, PLC ∫4, PLC ©1, PLC ©2, PLC ∂1, PLC ∂2 and PLC e. After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC ©2 associates with several tyrosine-phosphorylated proteins (Syk, SLP-76, Lyn, linker for activation of T cells (LAT) and the FcR © chain), which bind to its C-terminal SH2 domain. PLC ©1 associates with Syk in B cells, but PLC ©2 does not associate with Syk in platelets. The C-terminal SH2 domain is involved in the regulation of PLC ©2. In addition, Btk can induce PLC ©2 tyrosine phosphorylation and initiate calcium moblization in CD72-stimulated B lymphocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PLC γ2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of PLC &gamma 2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of PLC γ2 of human origin |
| Entrez Gene | 5336 |
|---|
Application
Western blot analysis of PLC γ2 expression in non-transfected 293T: (A), human PLC γ2 transfected 293T: (B) and MCF7 (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse lymph node frozen section showing membrane and cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing membrane and cytoplasmic localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lymphoma showing membrane and cytoplasmic staining.
Application
Western blot analysis of PLC γ2 expression in MCF7 (A), MCF7 + etoposide (B), U-937 (C) and THP-1 (D) whole cell lysates.| Application Notes | recommended for detection of PLC γ2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
