Application
Western blot analysis of MYH15 expression in human breast tissue extract.Application
Western blot analysis of MYH15 expression in non-transfected: (A) and human MYH15 transfected: (B) 293T whole cell lysates.| Product Name | MYH15 Antibody (E-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of myosin heavy chain 15 of human origin |
| Modification Notes | Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of two heavy chains (MHC) and four light chains (MLC) that interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Myosin heavy chains, encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of Myosin heavy chains are differentially expressed depending on the functional activity of the muscle. MYH15 (Myosin, heavy chain 15), is a 1,946 amino acid cytoplasmic protein that contains an N-terminal myosin head-like domain and one IQ domain. Involved in muscle contractions, the gene encoding MYH15 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of myosin heavy chain 15 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of myosin heavy chain 15 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of myosin heavy chain 15 of human origin |
| Entrez Gene | 22989 |
|---|
Application
Western blot analysis of MYH15 expression in human breast tissue extract.
Application
Western blot analysis of MYH15 expression in non-transfected: (A) and human MYH15 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of myosin heavy chain 15 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
