Application
Western blot analysis of αB-crystallin expression in non-transfected: (A) and human αB-crystallin transfected: (B) 293 whole cell lysates.Application
Western blot analysis of αB-crystallin expression in non-transfected: (A) and human αB-crystallin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of αB-crystallin expression in mouse eye (A) and rat eye (B) tissue extracts.Application
Immunofluorescence staining of methanol-fixed Y79 cells showing cytoplasmic localization.| Product Name | αB-crystallin Antibody (FL-175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-175 representing full length αB-crystallin of human origin |
| Modification Notes | Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into å, ∫ and © families, and the ∫- and ©-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, inclu-ding four homologous motifs, a connecting peptide, and N- and C-terminal extensions. å-crystallins consist of three gene products, åA-, åB- and åC-crystallin, which are members of the small heat shock protein family (HSP 20). å-crystallins act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, å-crystallins do not renature these proteins. Expression of åA-crystallin is restricted to the lens and defects of this gene cause the development of autosomal dominant congenital cataracts (ADCC). The human åB-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of åB-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of αB-crystallin and, to a lesser extent, αA-crystallin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-175 representing full length &alpha B-crystallin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-175 representing full length αB-crystallin of human origin |
| Entrez Gene | 1410 |
|---|
Application
Western blot analysis of αB-crystallin expression in non-transfected: (A) and human αB-crystallin transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of αB-crystallin expression in non-transfected: (A) and human αB-crystallin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of αB-crystallin expression in mouse eye (A) and rat eye (B) tissue extracts.
Application
Immunofluorescence staining of methanol-fixed Y79 cells showing cytoplasmic localization.| Application Notes | recommended for detection of αB-crystallin and, to a lesser extent, αA-crystallin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
