Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of TTP expression in non-transfected 293T: (A), human TTP transfected 293T: (B) and RAW 309 Cr.1 (C) whole cell lysates.Application
Western blot analysis of TTP expression in non-transfected 293T: (A), human TTP transfected 293T: (B) and RAW 309 Cr.1 (C) whole cell lysates.Application
Western blot analysis of human recombinant TTP fusion protein.| Product Name | TTP Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Tristetraprolin (TTP), also known as Nup475 and TIS11, is a zinc-binding protein encoded by the immediate-early response gene, Zfp-36 (1,2). Stimulation of quiescent fibroblasts by mitogens, including platelet derived growth factor and fibroblast growth factor, results in the serine phosphorylation of TTP and the rapid redistribution of the protein from the nucleus to the cytoplasm (3). In vitro studies have demonstrated that TTP is phosphorylated by p42 MAP kinase, indicating that the activity of TTP may be regulated by the MAP kinase pathway in vivo (4). Knockout mice deficient in TTP develop autoimmunity, inflammatory arthritis and dermatitis (5). These conditions can be reversed by blocking the activity of the inflammatory mediator, tumor necrosis factor-alpha (TNF-å), suggesting that TTP may function to negatively regulate the expression of TNF-a (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TTP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 166-285 mapping near the C-terminus of tristetrapolin (TTP) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 166-285 mapping near the C-terminus of tristetrapolin (TTP) of human origin |
| Entrez Gene | 7538 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of TTP expression in non-transfected 293T: (A), human TTP transfected 293T: (B) and RAW 309 Cr.1 (C) whole cell lysates.
Application
Western blot analysis of TTP expression in non-transfected 293T: (A), human TTP transfected 293T: (B) and RAW 309 Cr.1 (C) whole cell lysates.
Application
Western blot analysis of human recombinant TTP fusion protein.| Application Notes | recommended for detection of TTP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
