Application
Western blot analysis of NNMT expression in non-transfected (A) and human NNMT transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Product Name | NNMT Antibody (L-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Nicotinamide N-methyltransferase (NNMT) catalyzes the N-methylation of nicotin-amide and other pyridines. NNMT activity in the human liver has a bimodal frequency distribution, indicating that its enzyme activity may be modulated through a genetic polymorphism, which could have functional implications for individual differences in drug and xenobiotic toxicity. The gene that encodes human NNMT is approximately 16.5 kb in length, consists of three exons and two introns and maps to 11q23.2. NNMT isolated from the human liver was determined to be 969 nucleotides in length, with a 792 nucleotide open reading frame that encodes a 264 amino acid protein. The NNMT gene is presumed to be a significant genetic determinant of plasma homocysteine levels in Spanish families, since it encodes an enzyme involved in homocysteine synthesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of NNMT of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of NNMT of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 4837 |
|---|
Application
Western blot analysis of NNMT expression in non-transfected (A) and human NNMT transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
