Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of SULT2B1 expression in non-transfected: (A) and mouse SULT2B1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SULT2B1 expression in non-transfected: (A) and human SULT2B1 transfected: (B) 293T whole cell lysates.| Product Name | SULT2B1 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 296-365 mapping at the C-terminus of SULT2B1 of human origin |
| Modification Notes | The soluble sulfotransferases contribute to the elimination of xenobiotics, the activation of procarcinogens and the regulation of hormones. Members of the three groups comprising this superfamily show selectivity to certain substrate compounds. SULT1 sulfotransferases exhibit N-sulfating activities of carcinogenic heterocyclic amines, and are selective toward phenols, whereas SULT2 enzymes prefer hydroxysteroids and SULT3 family members are selective for N-substituted aryl and alicyclic compounds. SULT2A1 catalyzes the sulfonation of procarcinogen xenobiotics, hydroxysteroids and bile acids, and is highly expressed in adrenal and liver tissues. SULT2A1 plays a role in hepatic cholesterol homeostasis. SULT2B1 consists of two isoforms, SULT2B1a and SULT2B1b, which are transcribed from the same gene by alternative splicing of their first exons. Both isoforms are highly selective for the sulphation of 3b-hydroxysteroids such as pregnenolone, epiandrosterone, DHEA and androstenediol. SULT2B1b is expressed in prostate, skin, placenta and lung. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SULT2B1 isoforms 1 and 2 of mouse and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 296-365 mapping at the C-terminus of SULT2B1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 296-365 mapping at the C-terminus of SULT2B1 of human origin |
| Entrez Gene | 6820 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of SULT2B1 expression in non-transfected: (A) and mouse SULT2B1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SULT2B1 expression in non-transfected: (A) and human SULT2B1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of SULT2B1 isoforms 1 and 2 of mouse and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
