Application
Western blot analysis of claudin-2 expression in SW480 whole cell lysate.Application
Western blot analysis of claudin-2 expression in Hep G2 whole cell lysate.| Product Name | claudin-2 Antibody (A-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of claudin-2 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | The claudin superfamily consists of structurally related proteins that are important structural and functional components of tight junctions. Claudin-2, also known as CLDN2 or SP82, is a 230 amino acid multi-pass membrane protein that localizes to the cell junctions and belongs to the claudin superfamily. Able to form homopolymers of heteropolymers with other claudin family members, claudin-2 plays an essential role in mediating calcium-independent cell-adhesion activity that is necessary for tight junction-specific obliteration of the intercellular space. Overexpression of claudin-2 is associated with a variety of diseases, including lung cancer, colorectal cancer, gastrointestinal carcinomas and inflammatory bowel disease, further affirming the importance of claudin-2 in cell adhesion. The gene encoding claudin-2 maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of claudin-2 of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of claudin-2 of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 9075 |
|---|
Application
Western blot analysis of claudin-2 expression in SW480 whole cell lysate.
Application
Western blot analysis of claudin-2 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
