Application
Western blot analysis of SPA-1 expression in non-transfected: (A) and mouse SPA-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SPA-1 expression in non-transfected: (A) and human SPA-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SPA-1 expression in CCRF-CEM whole cell lysate.| Product Name | SPA-1 Antibody (M-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 691-990 mapping near the C-terminus of SPA-1 of mouse origin |
| Modification Notes | The SPA-1 (signal-induced proliferation-associated gene-1) protein is a principal RAP1 GTPase-activating protein in the hematopoietic progenitors and peripheral T cells. The SPA-1 gene is normally expressed in fetal and adult lymphohematopoietic tissues. Various types of mitogenic stimulation increase SPA-1 mRNA expression in normal lymphocytes. SPA-1 disrupts LFA-1-ICAM1-mediated adhesive interactions and subsequent T cell-receptor triggering and IL-2 production, possibly through inhibition of RAP1. Mice that are deficient for the SPA-1 gene develop age-dependent progression of T cell immunodeficiency followed by a spectrum of late onset myeloproliferative disorders, mimicking human chronic myeloid leukemia. SPA-1 also directly binds to AQP2 and plays a role in regulating AQP2 trafficking to the apical membrane. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SPA-1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 691-990 mapping near the C-terminus of SPA-1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 691-990 mapping near the C-terminus of SPA-1 of mouse origin |
| Entrez Gene | 6494 |
|---|
Application
Western blot analysis of SPA-1 expression in non-transfected: (A) and mouse SPA-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SPA-1 expression in non-transfected: (A) and human SPA-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SPA-1 expression in CCRF-CEM whole cell lysate.| Application Notes | recommended for detection of SPA-1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
