Application
Western blot analysis of AKAP 150 expression in C6 whole cell lysate (A) and rat cerebellum (B), rat brain (C) and mouse brain (D) tissue extracts.| Product Name | AKAP 150 Antibody (R-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 267-478 mapping to an internal region of AKAP 150 of rat origin |
| Modification Notes | The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates (1). Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. This family of proteins has been designated A-kinase anchoring proteins (AKAP) (1-3). Members of this family, including MAP2 (microtubule-associated protein 2), neuronally expressed AKAP 79 and AKAP 150, and the DNA binding AKAP 95, display differential tissue specificity and localization (4-6). Evidence suggests that AKAP 79 and AKAP 150 are both capable of anchoring PKA to postsynaptic densities (PSD), which are a network of proteins located on the internal surfaces of excitatory synapses (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AKAP 150 of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 267-478 mapping to an internal region of AKAP 150 of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 267-478 mapping to an internal region of AKAP 150 of rat origin |
| Entrez Gene | 238276 |
|---|
Application
Western blot analysis of AKAP 150 expression in C6 whole cell lysate (A) and rat cerebellum (B), rat brain (C) and mouse brain (D) tissue extracts.| Application Notes | recommended for detection of AKAP 150 of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
