Application
Western blot analysis of Sam 68 expression in non-transfected: (A) and mouse Sam 68 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Sam 68 expression in HeLa nuclear extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.| Product Name | Sam 68 Antibody (S-331) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Sam 68 is a protein that is phosphorylated on tyrosine and functions as a substrate for Src family tyrosine kinases during mitosis. Sam 68 also associates with several SH2 and SH3 domain-containing signaling proteins, such as GRB2 and PLC ©1. Originally cloned as Ras GAP-associated p62, further investigations have shown that Sam 68 and Ras GAP-associated p62 are not antigenically related, nor are they encoded by the same gene. Like Sam 68, the Sam 68-like mammalian proteins, SLM-1 and SLM-2, demonstrate RNA binding activity. Also like Sam 68, SLM-1 is tyrosine phosphorylated and functions as an adapter protein for signaling molecules, including GRB2, PLC ©1, Fyn and Ras GAP. SLM-2 is not tyrosine phosphorylated, nor does it appear to associate with GRB2, PLC ©1, Fyn or Ras GAP, indicating that SLM-2 may not be an adapter protein for these proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Sam 68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 331-443 mapping at the C-terminus of Sam 68 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 331-443 mapping at the C-terminus of Sam 68 of mouse origin |
| Entrez Gene | 10657 |
|---|
Application
Western blot analysis of Sam 68 expression in non-transfected: (A) and mouse Sam 68 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Sam 68 expression in HeLa nuclear extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.| Application Notes | recommended for detection of Sam 68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
