Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human GNMT transfected HEK 293T cells (B).Application
Western blot analysis of GNMT expression in non-transfected: (A) and human GNMT transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GNMT expression in non-transfected 293T: (A), human GNMT transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of GNMT expression in Hep G2 whole cell lysate.Application
Western blot analysis of GNMT expression in non-transfected 293T: (A), human GNMT transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | GNMT Antibody (H-225) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Glycine N-methyltransferase (GNMT) is a 295 amino acid protein that catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). This process indicates that GNMT probably plays a crucial role in the regulation of tissue concentration of AdoMet and in the metabolism of methionine. Originally identified as a methyl donor, AdoMet is now considered a key metabolite that regulates hepatocyte growth, death and differentiation. Biosynthesis of AdoMet occurs in all mammalian cells as the first step in methionine catabolism in a reaction catalyzed by methionine adenosyltransferase (MAT). Decreased hepatic AdoMet biosynthesis is a consequence of all forms of chronic liver injury. In chronic liver AdoMet deficiency, the liver is predisposed to further injury and can develop spontaneous steatohepatitis and hepatocellular carcinoma. However, impaired AdoMet metabolism, which occurs in patients with mutations of GNMT, can also lead to liver injury. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Glycine N-methyltransferase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 71-295 mapping at the C-terminus of GNMT of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 71-295 mapping at the C-terminus of GNMT of human origin |
| Entrez Gene | 27232 |
|---|
Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human GNMT transfected HEK 293T cells (B).
Application
Western blot analysis of GNMT expression in non-transfected: (A) and human GNMT transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GNMT expression in non-transfected 293T: (A), human GNMT transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of GNMT expression in Hep G2 whole cell lysate.
Application
Western blot analysis of GNMT expression in non-transfected 293T: (A), human GNMT transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of Glycine N-methyltransferase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
