Application
Western blot analysis of plakophilin 3 expression in non-transfected: (A) and human plakophilin 3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of plakophilin 3 expression in non-transfected 293T: (A), human plakophilin 3 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of plakophilin 3 expression in K-562 whole cell lysate.| Product Name | plakophilin 3 Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 3 (PKP3) is a 797 amino acid protein that contains eight arm-repeats and belongs to the beta-catenin family. Encoded by a gene that maps to human chromosome 11p15.5, plakophilin 3 localizes to desmosomes of most simple and nearly all stratified epithelia, as well as cell lines derived therefrom, with the exception of hepatocytes and hepatocellular carcinoma cells. Plakophilin 3 plays a role in both desmosome-dependent adhesion and signaling pathways, and may play a role in junctional plaques. Up-regulation of plakophilin 3 is a frequent and important feature of lung carcinogenesis, implicating plakophilin 3 as a candidate prognostic marker and therapeutic target for lung cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of plakophilin 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 51-220 mapping near the N-terminus of plakophilin 3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 51-220 mapping near the N-terminus of plakophilin 3 of human origin |
| Entrez Gene | 11187 |
|---|
Application
Western blot analysis of plakophilin 3 expression in non-transfected: (A) and human plakophilin 3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of plakophilin 3 expression in non-transfected 293T: (A), human plakophilin 3 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of plakophilin 3 expression in K-562 whole cell lysate.| Application Notes | recommended for detection of plakophilin 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
