Application
Western blot analysis of c-Jun expression in non-transfected 293T: (A), human c-Jun transfected 293T: (B) and UV irradiated HeLa (C) whole cell lysates.Application
Western blot analysis of c-Jun expression in non-transfected CHO: (A), human c-Jun transfected CHO: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of Jun D expression in non-transfected: (A) and mouse Jun D transfected: (B) 293T whole cell lysates and NIH/3T3 nuclear extract (C).Application
Western blot analysis of c-Jun expression in non-transfected 293T: (A), mouse c-Jun transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of c-Jun expression in non-transfected: (A) and mouse c-Jun transfected: (B) 293T whole cell lysates.Application
Western blot analysis of c-Jun expression in non-transfected: (A) and mouse c-Jun transfected: (B) 293T whole cell lysates.Application
Western blot analysis of c-Jun expression in non-transfected: (A) and human c-Jun transfected: (B) 293 whole cell lysates.Application
Western blot analysis of c-Jun expression in non-transfected (A) and human c-Jun transfected (B) CHO whole cell lysates.Application
Western blot analysis of c-Jun expression in nontransfectedcontrol (A) and c-Jun siRNA transfected (B) NIH/3T3 cells. Blot probed with c-Jun: . GAPDH (FL-335): used as specificity and loading control.Application
10% (A,C) and 1% (B,D) of input DNAs were amplified. Antibodies used included c-Jun (D): ; c-Fos (6-2H-2F): 7 and Ets-1/Ets-2 (C-275): . Data kindly provided by J. Imbert and reproduced with permission from Yeh, J.H., et al, Mol. Cell. Biol., 2001, 21:4515-4527.Application
Western blot analysis of c-Jun expression in Jurkat nuclear extract.Application
Antibodies tested include c-Jun (D): (A,B) and c-Jun (N): (C,D).| Product Name | c-Jun Antibody (D) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of c-Jun, Jun B and Jun D p39 proteins of mouse, rat, human, avian and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping a highly conserved DNA binding domain of c-Jun p39 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping a highly conserved DNA binding domain of c-Jun p39 of mouse origin |
| Entrez Gene | 3725 |
|---|
Application
Western blot analysis of c-Jun expression in non-transfected 293T: (A), human c-Jun transfected 293T: (B) and UV irradiated HeLa (C) whole cell lysates.
Application
Western blot analysis of c-Jun expression in non-transfected CHO: (A), human c-Jun transfected CHO: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of Jun D expression in non-transfected: (A) and mouse Jun D transfected: (B) 293T whole cell lysates and NIH/3T3 nuclear extract (C).
Application
Western blot analysis of c-Jun expression in non-transfected 293T: (A), mouse c-Jun transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of c-Jun expression in non-transfected: (A) and mouse c-Jun transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of c-Jun expression in non-transfected: (A) and mouse c-Jun transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of c-Jun expression in non-transfected: (A) and human c-Jun transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of c-Jun expression in non-transfected (A) and human c-Jun transfected (B) CHO whole cell lysates.
Application
Western blot analysis of c-Jun expression in nontransfectedcontrol (A) and c-Jun siRNA transfected (B) NIH/3T3 cells. Blot probed with c-Jun: . GAPDH (FL-335): used as specificity and loading control.
Application
10% (A,C) and 1% (B,D) of input DNAs were amplified. Antibodies used included c-Jun (D): ; c-Fos (6-2H-2F): 7 and Ets-1/Ets-2 (C-275): . Data kindly provided by J. Imbert and reproduced with permission from Yeh, J.H., et al, Mol. Cell. Biol., 2001, 21:4515-4527.
Application
Western blot analysis of c-Jun expression in Jurkat nuclear extract.
Application
Antibodies tested include c-Jun (D): (A,B) and c-Jun (N): (C,D).| Application Notes | recommended for detection of c-Jun, Jun B and Jun D p39 proteins of mouse, rat, human, avian and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
