Application
Western blot analysis of BMPR-IA expression in DU 145 (A) and T98G (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of BMPR-IA expression in non-transfected: (A) and human BMPR-IA transfected: (B) 293T whole cell lysates.Application
Western blot analysis of BMPR-IA expression in PC-3 whole cell lysate.Application
Western blot analysis of BMPR-IA expression in HeLa (A) and Jurkat (B) whole cell lysates.| Product Name | BMPR-IA Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 24-83 of BMPR-IA of human origin |
| Modification Notes | Members of the transforming growth factor b superfamily bind to a pair of transmembrane proteins, known as receptor types I and II, which contain serine/threonine kinases and associate to form a signaling complex (1). Two type I receptors have been characterized, BMPR-IA (also designated SKR5, ALK-3, and BRK-1) and BMPR-IB (also designated ALK-6 and SKR 6), that bind to bone morphogenetic proteins (BMP)-2, BMP-4 and osteogenic protein (OP)-1 (also designated BMP-7) (1–4). BMPR-IA and BMPR-IB are both expressed in human glioma cell lines (3). The type II receptor, BMPR-II, efficiently binds to OP-1 and BMP-2 and weakly binds BMP-4, and it is widely expressed in different tissues, including brain (1,5,6). The BMP receptor family members are thought to mediate distinct effects on gene expression, cell differentiation, and morphogenesis in a dose dependent fashion (1,5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of BMPR-IA of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 24-83 of BMPR-IA of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 24-83 of BMPR-IA of human origin |
| Entrez Gene | 657 |
|---|
Application
Western blot analysis of BMPR-IA expression in DU 145 (A) and T98G (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of BMPR-IA expression in non-transfected: (A) and human BMPR-IA transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of BMPR-IA expression in PC-3 whole cell lysate.
Application
Western blot analysis of BMPR-IA expression in HeLa (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of BMPR-IA of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
