Application
Western blot analysis of Tyro3 expression in non-transfected: (A) and human Tyro3 transfected: (B) 293T whole cell lysates.| Product Name | Tyro3 Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 781-890 mapping at the C-terminus of Tyro3 of human origin |
| Modification Notes | Receptor tyrosine kinases (RTKs) represent an important class of transmembrane signaling molecules. Binding of the extracellular domain of an RTK to its cognate ligand leads to receptor dimerization and the activation of the intrinsic tyrosine kinase activity of its intracellular kinase domain. The Axl/UFO subfamily of receptor tyrosine kinases is comprised of members Tyro3 (also referred to as BYK, Brt, Dtk, RSE, Tif or Sky), Axl (also called Tyro7 or UFO) and Mer (also called Nyk, c-Eyk and Tyro12). Members of this family have a common molecular structure which contains an N-terminal extracellular domain comprised of two Ig domains, two FNIII domains and a membrane spanning single helix followed by the cytoplasmic tyrosine kinase domain. These RTKs are functionally significant in spermatogenesis, immunoregulation and phagocytosis. Tyro3, Axl and Mer are widely expressed in adult tissues with their expression most abundant in brain, testis, lymphatic and vascular tissue. Tyro3 has been shown to undergo posttranslational modifications including both tyrosine phosphorylation as well as glycosylation. Two proteins, Protein S and Gas6, have been proposed as ligands for the Axl/UFO family of receptors. Both function as anti-coagulants through an unknown mechanism. Gas6 was cloned as a growth arrest-specific gene, while Protein S is an abundant serum protein which is thought to act by indirectly inhibiting proteases involved in the coagulation response. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Tyro3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 781-890 mapping at the C-terminus of Tyro3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 781-890 mapping at the C-terminus of Tyro3 of human origin |
| Entrez Gene | 7301 |
|---|
Application
Western blot analysis of Tyro3 expression in non-transfected: (A) and human Tyro3 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Tyro3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
