Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of HGFα expression in c4 whole cell lysate.Application
Western blot analysis of human recombinant HGF.| Product Name | HGFα Antibody (H-145) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 32-176 mapping near the N-terminus of HGFα of human origin |
| Modification Notes | Hepatocyte growth factor, or HGF, is a pleiotropic growth factor variously designated as scatter factor, hematopoietin A and mammary growth factor (1,2). HGF is synthesized as a single chain, 728 amino acid precursor with a 29 amino acid signal peptide which is not present in the mature protein (3). Biologically active HGF is composed of a disulfide linked å chain and a ∫ chain, both of which are highly glycosylated (4). HGF exerts its biological effects through the HGF receptor, c-Met, which is expressed by normal hepatocytes, gastric and intestinal epithelium, ovarian and endometrial endothelium and in the basal layers of skin (4,5). While c-Met is not thought to be expressed in normal lung, thyroid or pancreatic tissue, c-Met has been detected in tumors originating from such tissue (6). The c-Met proto-oncogene encodes a 1408 amino acid glycoprotein that represents the prototypic member of a novel family of receptor tyrosine kinases (RTKs) that include Ron, Sea and Sex (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HGFα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 32-176 mapping near the N-terminus of HGF&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 32-176 mapping near the N-terminus of HGFα of human origin |
| Entrez Gene | 3082 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of HGFα expression in c4 whole cell lysate.
Application
Western blot analysis of human recombinant HGF.| Application Notes | recommended for detection of HGFα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
