Application
Western blot analysis of Paip1 expression in non-transfected 293T: (A), human Paip1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Paip1 expression in non-transfected 293T: (A), human Paip1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Paip1 expression in non-transfected: (A) and truncated mouse Paip1 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.| Product Name | Paip1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 180-479 mapping at the C-terminus of Paip1 of human origin |
| Modification Notes | Paip, for PABP-interacting protein, binds to the polyadenylate-binding protein (PABP), which in yeasts and plants has been shown to bind to the eukaryotic initiation factor component eIF4G. There are two Paip proteins called Paip1 and Paip2. Paip1 stimulates translation, and Paip2, which competes with Paip1 for binding to PABP, represses translation. Paip2 decreases the affinity of PABP for polyadenylate RNA, and disrupts the repeating structure of poly(A) ribonucleoprotein. Paip1 contains an eIF4A-binding region and a proline-rich N terminus. Overexpression of Paip1 in COS-7 cells stimulates translation, perhaps by providing a physical link between the mRNA termini. The human Paip1 gene encodes a 480 amino acid protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Paip1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 180-479 mapping at the C-terminus of Paip1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 180-479 mapping at the C-terminus of Paip1 of human origin |
| Entrez Gene | 10605 |
|---|
Application
Western blot analysis of Paip1 expression in non-transfected 293T: (A), human Paip1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Paip1 expression in non-transfected 293T: (A), human Paip1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Paip1 expression in non-transfected: (A) and truncated mouse Paip1 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.| Application Notes | recommended for detection of Paip1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
