Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing membrane and cytoplasmic staining of squamous epithelial cells.| Product Name | periplakin Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 41-240 mapping near the N-terminus of periplakin of human origin |
| Modification Notes | Paraneoplastic pemphigus (PNP) is an autoimmune blistering disease that is associated with underlying neoplasms. PNP sera reacts with multiple plakin family proteins, among which only envoplakin and periplakin are constantly detected. Periplakin, a membrane-associated precursor of the epidermal cornified envelope and desmosomes, is a member of the plakin family of proteins. The periplakin gene PPL is mapped to human chromosome 16p13.3, and its promoter contains multiple AP2 sites and a SP1 site. Periplakin localizes to desmosomes, the interdesomosomal plasma membrane and intermediate filaments. The periplakin rod domain is necessary for the redistribution of envoplakin to desmosomes and the cytoskeleton by heterodimerization. The periplakin linker domain is required for intermediate filament association. The N-terminus of periplakin accumulates at the cell surface of microvilli in association with cortical actin. Envoplakin and periplakin localize independently to desmosomes, and the distribution of envoplakin at the interdesmosomal plasma membrane depends on heterodimerization with periplakin. The N-terminus of periplakin, therefore, plays an important role in forming the scaffold on which the cornfied envelop is assembled. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of periplakin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 41-240 mapping near the N-terminus of periplakin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 41-240 mapping near the N-terminus of periplakin of human origin |
| Entrez Gene | 5493 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing membrane and cytoplasmic staining of squamous epithelial cells.| Application Notes | recommended for detection of periplakin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
