Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.Application
Western blot analysis of casein kinase IIα expression in non-transfected 293: (A), human casein kinase IIα transfected 293: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of casein kinase IIα expression in non-transfected 293T: (A), mouse casein kinase IIα transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of casein kinase IIα expression in non-transfected: (A), human casein kinase IIα transfected: (B) and human casein kinase IIα transfected: (C) 293T whole cell lysates.Application
Western blot analysis of casein kinase IIα expression in non-transfected: (A) and mouse casein kinase IIα transfected: (B) 293T whole cell lysates.| Product Name | casein kinase IIα Antibody (H-286) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 2-320 of casein kinase IIα of human origin |
| Modification Notes | Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Iå, I©, I∂ and Ie, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an å2∫2 or an åå'∫2 structure. The a catalytic subunit is stimulated by the ∫ regulatory subunit, which undergoes autophosphorylation. Casein kinase II activity is high in the cytosol and nucleus of proliferating and differentiating cells. Casein kinase II is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of casein kinase IIa and casein kinase IIα' of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2-320 of casein kinase II&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2-320 of casein kinase IIα of human origin |
| Entrez Gene | 1457 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.
Application
Western blot analysis of casein kinase IIα expression in non-transfected 293: (A), human casein kinase IIα transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of casein kinase IIα expression in non-transfected 293T: (A), mouse casein kinase IIα transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of casein kinase IIα expression in non-transfected: (A), human casein kinase IIα transfected: (B) and human casein kinase IIα transfected: (C) 293T whole cell lysates.
Application
Western blot analysis of casein kinase IIα expression in non-transfected: (A) and mouse casein kinase IIα transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of casein kinase IIa and casein kinase IIα' of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
