Application
Western blot analysis of selenocysteine lyase expression in non-transfected: (A) and human selenocysteine lyase transfected: (B) 293T whole cell lysates.Application
Western blot analysis of selenocysteine lyase expression in non-transfected: (A) and mouse selenocysteine lyase transfected: (B) 293T whole cell lysates.| Product Name | selenocysteine lyase Antibody (H-155) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 291-368 mapping within an internal region of selenocysteine lyase of human origin |
| Modification Notes | Selenocysteine lyase (SCL) catalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium. The reaction depends on the presence of pyridoxal 5’-phosphate as a cofactor, and occurs in liver, kidney, heart, adrenal and muscle tissue. This regulation by the 5’phosphate resembles the regulatory mechanisms for other enzymes, including aspartate beta-decarboxylase, arginine racemase and kynureninase. SCL potentially functions as a selenium delivery protein to selenophosphate synthetase, facilitating selenoprotein biosynthesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| recommended for detection of selenocysteine lyase of mouse, rat and human origin by WB, IP and IF also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 291-368 mapping within an internal region of selenocysteine lyase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 291-368 mapping within an internal region of selenocysteine lyase of human origin |
| Entrez Gene | 51540 |
|---|
Application
Western blot analysis of selenocysteine lyase expression in non-transfected: (A) and human selenocysteine lyase transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of selenocysteine lyase expression in non-transfected: (A) and mouse selenocysteine lyase transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of selenocysteine lyase of mouse, rat and human origin by WB, IP and IF also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
