Application
Western blot analysis of JIK expression in non-transfected (A) and human JIK transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic and membrane localization.| Product Name | JIK Antibody (D-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of JIK of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | JNK/SAPK-inhibitory kinase (JIK) is a serine/threonine kinase that belongs to the STE20 kinase family. The kinase domain of JIK is similar to the GCK-like subfamily of STE20 kinases, while its non-catalytic domain is similar to a Caenorhabditis elegans putative serine/threonine kinase, SULU. JIK inhibits c-Jun NH2-terminal kinase/stress-activated protein kinase (JNK/SAPK), which is activated by many types of cellular stresses and extracellular signals. JNK/SAPK regulates cell survival, apoptosis and proliferation in mouse development. JIK is negatively regulated by epidermal growth factor (EGF) and tyrosine kinase receptors. In unstimulated human T cells, JIK is cytoplasmic, whereas in the continuously dividing human T cells of Jurkat lymphoma, JIK is nuclear. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of JIK of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of JIK of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 51347 |
|---|
Application
Western blot analysis of JIK expression in non-transfected (A) and human JIK transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic and membrane localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
