Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing membrane and cytoplasmic staining of glandular cells.Application
Western blot analysis of Mesothelin expression in non-transfected: (A) and human Mesothelin transfected: (B) 293T whole cell lysates.| Product Name | Mesothelin Antibody (H-280) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 295-574 mapping near the C-terminus of Mesothelin of human origin |
| Modification Notes | Mesothelin is a glycosylphosphatidylinositol-linked cell-surface molecule expressed in the mesothelial lining of the body cavities and in many tumor cells. Mesothelin is a tumor antigen on the surface of human ovarian cancers and mesotheliomas. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid, and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. In adult mouse tissues the mesothelin transcript is present in lung, heart, spleen, liver, kidney and testis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Mesothelin of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 295-574 mapping near the C-terminus of Mesothelin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 295-574 mapping near the C-terminus of Mesothelin of human origin |
| Entrez Gene | 10232 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing membrane and cytoplasmic staining of glandular cells.
Application
Western blot analysis of Mesothelin expression in non-transfected: (A) and human Mesothelin transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Mesothelin of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
