| Product Name | Cdc53 Antibody (y-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 516-815 mapping at the C-terminus of Cdc53 of Saccharomyces cerevisiae origin |
| Modification Notes | Cell cycle progression is controlled at a point late in G1 designated Start. Passage through Start requires the activity of the cyclin-dependent protein kinase Cdc28 (1,2). Transition from G1 to S phase requires the association of Cdc28 with members of the G1 cyclin family (3). This progression also requires the destruction of the S-phase cyclin/Cdk inhibitor, Sic1 (4). Sic1 proteolysis is mediated in part by the ubiquitin-conjugating enzyme Cdc34 (5). Cdc4, a potential ubiquitin-protein ligase, is also involved in the degradation of Sic1 (6). Another protein thought to play a role in the ubiquitin-protein ligase complex is Cdc53. This protein binds to Cdc34 and targets phosphorylated G1 cyclins for ubiquitin-mediated degradation (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Cdc53 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 516-815 mapping at the C-terminus of Cdc53 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 516-815 mapping at the C-terminus of Cdc53 of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of Cdc53 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
