Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of MTF-1 expression in non-transfected: (A) and mouse MTF-1 transfected: (B) 293T whole cell lysates and mouse heart tissue extract (C).| Product Name | MTF-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 454-753 mapping at the C-terminus of MTF-1 of human origin |
| Modification Notes | The metal-responsive element (MRE)-binding transcription factor (MTF-1) stimulates the expression of metallothioneins in response to the exposure of cells to heavy metals (1-3). MTF-1 contains six zinc fingers in the DNA binding domain (2-3). The phosphorylation of MTF-1 in response to metal exposure appears to play a significant role in the ability of MTF-1 to activate metallothionein transcription (4). In addition to its role in metallothionein activation, MTF-1 is involved in a post-transcription regulatory complex for ribosomal protein S25 (5). MTF-1, La and p53 inhibit the nuclear export of S25 mRNA in response to nutrient depravation (5). Furthermore, MTF-1 acts as a chromatin insulator on integrated transgenes in cultured cells to insulate active loci against chromatin silencing (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of MTF-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 454-753 mapping at the C-terminus of MTF-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 454-753 mapping at the C-terminus of MTF-1 of human origin |
| Entrez Gene | 4520 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of MTF-1 expression in non-transfected: (A) and mouse MTF-1 transfected: (B) 293T whole cell lysates and mouse heart tissue extract (C).| Application Notes | recommended for detection of MTF-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
