| Product Name | Smt3 Antibody (y-84) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 15-98 of Smt3 of Saccharomyces cerevisiae origin |
| Modification Notes | Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins. The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign, and improperly folded proteins by targeting them for proteosome degradation. In Saccharomyces cerevisiae, ubiquitin-like proteins include Rub1, Ula1, Uba3, Smt3, Ubc2, Ubc12 and Ubc9. Rub1 shares 53% homology with ubiquitin and requires activation via Ula1, Uba3 and Ubc12 in order to conjugate to substrates directed to different proteolytic systems. Smt3, which is similar to mammalian SUMO-1, requires Ubc9 for conjugation to other proteins. Skp1 connects cell cycle regulators to the ubiquitin proteolysis machinery. Hrt1 is an essential subunit of Skp1p-cullin-F-box (SCF) complexes, which are necessary for the degradation of various regulatory proteins. Ubc13 forms a complex with Mms2 that is involved the error-free DNA postreplication repair (PRR) pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Smt3 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 15-98 of Smt3 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 15-98 of Smt3 of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of Smt3 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
