Application
Western blot analysis of yeast recombinant Yap1 fusion protein.| Product Name | Yap1 Antibody (y-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 351-650 mapping at the C-terminus of Yap1 of Saccharomyces cerevisiae origin |
| Modification Notes | Commitment to cell division occurs at a point late in the G1 phase of the cell cycle, termed Start. Passage through Start requires the activation of the Cdc28 protein kinase by the cell cycle-regulated G1 cyclins (1). Maximal expression of these G1 cyclins is induced by the heterodimeric transcription factor complex composed of Swi4 (also designated Art1) and Swi6 (2). Swi4 is the DNA-binding subunit of this complex (3). In addition to binding Swi4, Swi6 forms a complex with Mbp1. This complex activates S-phase cyclins and genes involved in DNA synthesis (4) Rpb1 is the largest subunit of the yeast RNA polymerase II (5). Srb4 is a basal transcription factor that is essential for the establishment of the transcription initiation apparatus (5). Stress factors induce transcription through the induction of various transcription factors. Yap1 activates expression in response to oxidative stress, while Msn2 and Msn4 mediate transcription via the stress response element (STRE) (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Yap1 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 351-650 mapping at the C-terminus of Yap1 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 351-650 mapping at the C-terminus of Yap1 of Saccharomyces cerevisiae origin |
Application
Western blot analysis of yeast recombinant Yap1 fusion protein.| Application Notes | recommended for detection of Yap1 of Saccharomyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
