Application
Western blot analysis of rapsyn expression in Hs 732.Sk/Mu whole cell lysate (A) and mouse skeletal muscle tissue extract (B).Application
Western blot analysis of rapsyn expression in A-673 (A) and HeLa (B) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Product Name | rapsyn Antibody (H-205) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 178-382 mapping near the C-terminus of rapsyn of human origin |
| Modification Notes | The RAPSN gene locus is located on chromosome 11p11.1-p11.2 and encodes a peripheral membrane protein. Rapsyn (receptor-associated protein of the synapse) is expressed in the postsynaptic membrane of skeletal muscle. Rap-syn is required for the clustering of nicotinic acetylcholine receptors (nAChR). Rapsyn self-associates through at least two of its seven tetra-tricopeptide repeats (TPRs). Rapsyn interacts with the large intracellular domain of the nAChR a subunit through the hydrophobic surface of the coiled-coil domain. Rapsyn modifies trafficking of AChR within the cell. Expression is essential for agrin-induced AChR clustering. Overexpression inhibits agrin-induced AChR clustering pathway. Absence of rapsyn causes deficit in the formation of postsynaptic specializations at neuromuscular synapse, which increases axonal branching and motonueron survival. Rapsyn plays a role in selective targeting of newly synthesized intracellular AChR to postsynaptic membrane. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of rapsyn of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 178-382 mapping near the C-terminus of rapsyn of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 178-382 mapping near the C-terminus of rapsyn of human origin |
| Entrez Gene | 5913 |
|---|
Application
Western blot analysis of rapsyn expression in Hs 732.Sk/Mu whole cell lysate (A) and mouse skeletal muscle tissue extract (B).
Application
Western blot analysis of rapsyn expression in A-673 (A) and HeLa (B) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Application Notes | recommended for detection of rapsyn of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
