Application
Western blot analysis of PRP6 expression in non-transfected: (A) and mouse PRP6 transfected: (B) 293T whole cell lysates and K-562 (C) and HL-60 (D) nuclear extracts.Application
Western blot analysis of PRP6 expression in K-562 (A) and HL-60 (B) nuclear extracts.| Product Name | PRP6 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PRP6 of human origin |
| Modification Notes | Assembly of pre-mRNA spliceosomes requires the interaction between snRNPs U4/U6 and U5 to form the [U4/U6.U5] tri-snRNP. In yeast, the small nuclear ribonucleoprotein-associating protein, Prp6p is necessary for the accumulation of the [U4/U6.U5] tri-snRNP. Yeast Prp6p is uniquely located in discrete subnuclear regions, similar to the subnuclear localization of mammalian splicing components. Isolated from HeLa nuclear extract, mammalian PRP6 shares conserved tetrarico peptide repeats with yeast Prp6p, making PRP6 the mammalian homolog of yeast Prp6p. In contrast to yeast Prp6p, which is specific for U4/U6, the human PRP6 interacts within the tri-snRNP with both the U5 and the U4/U6 snRNPs via protein-protein interactions, thus providing a bridge that connects the two snRNP particles. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of PRP6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PRP6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of PRP6 of human origin |
| Entrez Gene | 24148 |
|---|
Application
Western blot analysis of PRP6 expression in non-transfected: (A) and mouse PRP6 transfected: (B) 293T whole cell lysates and K-562 (C) and HL-60 (D) nuclear extracts.
Application
Western blot analysis of PRP6 expression in K-562 (A) and HL-60 (B) nuclear extracts.| Application Notes | recommended for detection of PRP6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
