Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of subset of cells in seminiferous ducts.Application
Western blot analysis of Geminin expression in non-transfected 293T: (A), mouse Geminin transfected 293T: (B) and MM-142 (C) whole cell lysates.| Product Name | Geminin Antibody (FL-209) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-209 representing full length Geminin of human origin |
| Modification Notes | Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle (1). DNA replication requires the coordinated association of cdc6 and minichromosome maintenance (MCM) proteins with chromatin (2). Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring (1,3). Expression of geminin fluctuates throughout the cell cycle with geminin levels lowest at G1. Throughout S, G2 and M phases, geminin levels are consistently elevated followed by a decrease during mitosis (1,4). The initiation of DNA replication is dependent on the the degradation of geminin during mitosis and the absence of geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis (5, 6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Geminin by mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-209 representing full length Geminin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-209 representing full length Geminin of human origin |
| Entrez Gene | 51053 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of subset of cells in seminiferous ducts.
Application
Western blot analysis of Geminin expression in non-transfected 293T: (A), mouse Geminin transfected 293T: (B) and MM-142 (C) whole cell lysates.| Application Notes | recommended for detection of Geminin by mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
