Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Western blot analysis of β-Arrestin-1/2 expression in SK-N-MC whole cell lysate.Application
Western blot analysis of β-Arrestin-2 expression in non-transfected: (A) and human β-Arrestin-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of β-Arrestin-2 expression in non-transfected: (A) and human β-Arrestin-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of β-Arrestin-1/2 expression in rat skeletal muscle tissue extract.| Product Name | β-Arrestin-1/2 Antibody (H-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 7-290 mapping at the N-terminus of β-Arrestin-1 of human origin |
| Modification Notes | The members of the G protein coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors (1). The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds (2,3). Members of the ∫-Arrestin family regulate receptor binding to G proteins (4). ∫-Arrestins have been found to be located at postsynaptic sites, where they are thought to act in concert with ∫ARK (∫ARK1, also designated GRK 2, or ∫ARK2, also designated GRK 3) to regulate G protein-coupled neurotransmitter receptors. Expression of ∫-Arrestin-1 and b-Arrestin-2 is seen predominantly in spleen and neuronal tissues (5). It has been shown that ∫-Arrestin-1 expression is modulated by intracellular cAMP, which may be a novel mechanism for the regulation of receptor-mediated responses (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of β-Arrestin-1, and -2, and, to a lesser extent, S-Arrestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 7-290 mapping at the N-terminus of &beta -Arrestin-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 7-290 mapping at the N-terminus of β-Arrestin-1 of human origin |
| Entrez Gene | 408 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Western blot analysis of β-Arrestin-1/2 expression in SK-N-MC whole cell lysate.
Application
Western blot analysis of β-Arrestin-2 expression in non-transfected: (A) and human β-Arrestin-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of β-Arrestin-2 expression in non-transfected: (A) and human β-Arrestin-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of β-Arrestin-1/2 expression in rat skeletal muscle tissue extract.| Application Notes | recommended for detection of β-Arrestin-1, and -2, and, to a lesser extent, S-Arrestin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
