Application
Western blot analysis of CYP11B1/2 expression in MCF7 whole cell lysate.Application
Western blot analysis of CYP11B1/2 expression in rat adrenal gland tissue extract.| Product Name | CYP11B1/2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 204-503 (deletion 401-466) mapping at the C-terminus of CYP11B1 of human origin |
| Modification Notes | The steroid 11beta-hydroxylase gene, also designated Cyp11b-1, is a marker for the functional differentiation of cells in the zonae fasciculata reticularis. The deduced protein CYP11B1 consists of 466 amino acids containing a secretory signal, epidermal growth factor-like repeats, and a proteolytically inactive cathepsin B-related sequence. A related protein, human aldosterone synthase (CYP11B2), is involved in substrate recognition and conversion, with a functionally significant residue 112 in the N-terminal region of human CYP11B2. The inherited disorder glucocorticoid-remediable aldosteronism iscaused by a chimeric gene duplication between the CYP11B1 and CYP11B2 genes. This disorder is characterized by hyperaldosteronism and high levels of 18-hydroxycortisol and 18-oxocortisol, which are under ACTH control. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CYP11B1 and CYP11B2 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 204-503 (deletion 401-466) mapping at the C-terminus of CYP11B1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 204-503 (deletion 401-466) mapping at the C-terminus of CYP11B1 of human origin |
Application
Western blot analysis of CYP11B1/2 expression in MCF7 whole cell lysate.
Application
Western blot analysis of CYP11B1/2 expression in rat adrenal gland tissue extract.| Application Notes | recommended for detection of CYP11B1 and CYP11B2 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
