Application
Western blot analysis of Agrin expression in non-transfected control (A) and Agrin siRNA transfected (B) LADMAC cells. Blot probed with Agrin (H-300): . α-actinin (H-2): used as specificity and loading control.| Product Name | Agrin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1727-2026 mapping at the C-terminus of Agrin of human origin |
| Modification Notes | Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation (1-3). Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction (4,5). Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated “y” and “z” display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly (5). Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention (6). Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation (7). Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Agrin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1727-2026 mapping at the C-terminus of Agrin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1727-2026 mapping at the C-terminus of Agrin of human origin |
| Entrez Gene | 375790 |
|---|
Application
Western blot analysis of Agrin expression in non-transfected control (A) and Agrin siRNA transfected (B) LADMAC cells. Blot probed with Agrin (H-300): . α-actinin (H-2): used as specificity and loading control.| Application Notes | recommended for detection of Agrin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
