Application
Western blot analysis of γ-crystallin expression in rat eye tissue extract.| Product Name | γ-crystallin Antibody (FL-175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-175 representing full length γB-crystallin of human origin |
| Modification Notes | Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into å, ∫, and © families, and the ∫ and ©-crystallins also comprise a superfamily. Crystallins usually contain seven distinctive protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. ©-crystallins are structural proteins in the lens, and they exists as monomers which typically lack connecting peptides and terminal extensions. The ©-crystallins include seven closely related ©A, ©B, ©C, ©D, ©E, ©F, and ©G-crystallin, as well as the ©N and ©S-crystallin genes. The ©-crystallins are differentially regulated after early development, and are involved in cataract formation as a result of either age-related protein degradation or genetic mutation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of γA-, γB-, γC-, γD-, γE- and γF-crystallin and, to a lesser extent, γS-crystallin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-175 representing full length &gamma B-crystallin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-175 representing full length γB-crystallin of human origin |
| Entrez Gene | 1419 |
|---|
Application
Western blot analysis of γ-crystallin expression in rat eye tissue extract.| Application Notes | recommended for detection of γA-, γB-, γC-, γD-, γE- and γF-crystallin and, to a lesser extent, γS-crystallin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
