Application
Western blot analysis of HAI-1 expression in non-transfected: (A) and mouse HAI-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HAI-1 expression in non-transfected 293T: (A), human HAI-1 transfected 293T: (B) and human platelet (C) whole cell lysates.Application
Western blot analysis of HAI-1 expression in non-transfected: (A) and mouse HAI-1 transfected: (B) 293T whole cell lysates.| Product Name | HAI-1 Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 36-215 mapping near the N-terminus of HAI-1 of human origin |
| Modification Notes | Tissue damage, such as hepatic and renal injury, initiates hepatocyte growth factor activator (HGFAC)-mediated limited proteolytic activation of the inactive single-chain precursor form of HGF. Initially, HGFAC is produced as a precursor protein, which is activated by limited proteolysis and is neutralized by specific inhibitors known as HGF activator inhibitors, designated HAIs. HAIs belong to the Kunitz-type serine protease inhibitor family. HAIs target HGF activator and are involved in the regulation of proteolytic activation of HGF in injured tissues. Human HAI-1 transcript is expressed in various human tissues, such as adult placenta, kidney, pancreas, prostate and small intestine, and fetal kidney and lung. It translates into a 478 amino acid protein. The human HAI-2 gene maps to chromosome 19q13.1 and encodes a 252 amino acid protein, also designated human placental bikunin or kop (Kunitz domain containing protein over-expressed in pancreatic cancer). HAI-1 and HAI-2 are produced in membrane-associated forms, which are secreted as active, proteolytically truncated proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HAI-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 36-215 mapping near the N-terminus of HAI-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 36-215 mapping near the N-terminus of HAI-1 of human origin |
| Entrez Gene | 6692 |
|---|
Application
Western blot analysis of HAI-1 expression in non-transfected: (A) and mouse HAI-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HAI-1 expression in non-transfected 293T: (A), human HAI-1 transfected 293T: (B) and human platelet (C) whole cell lysates.
Application
Western blot analysis of HAI-1 expression in non-transfected: (A) and mouse HAI-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of HAI-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
