Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts.Application
Western blot analysis of DJ-1 expression in non-transfected 293T: (A), mouse DJ-1 transfected 293T: (B) and SK-N-SH (C) whole cell lysates.Application
Western blot analysis of DJ-1 expression in HeLa whole cell lysate.Application
Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic staining.Application
Western blot analysis of DJ-1 expression in BT-20 (A) and HeLa (B) whole cell lysates.| Product Name | DJ-1 Antibody (FL-189) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-189 representing full length DJ-1 of human origin |
| Modification Notes | The DJ-1 gene encodes a highly-conserved protein which is implicated in a number of cell processes. DJ-1 was first identified as a novel oncogene that transformed mouse NIH/3T3 cells in cooperation with activated Ras. Additionally, DJ-1 was cloned in rat as SP22 or CAP-1, and found to be an infertility-related sperm protein whose expression is reduced in sperm treated with toxicants. DJ-1 also positively regulates the androgen receptor (AR) by forming a complex with PIASxå, a negative regulator of AR. The gene encoding human DJ-1 maps to chromosome 1p36.23, a region identified as a hot spot of chromosome abnormalities in several tumor cells. Subsequently, mutations in the DJ-1 gene have been implicated in Parkinson’s disease, and loss of DJ-1 function leads to neurodegeneration. DJ-1 is a ubiquitously expressed protein that is induced in response to growth stimuli and translocates from the cytoplasm to the nucleus during the S phase of the cell cycle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of DJ-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-189 representing full length DJ-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-189 representing full length DJ-1 of human origin |
| Entrez Gene | 11315 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts.
Application
Western blot analysis of DJ-1 expression in non-transfected 293T: (A), mouse DJ-1 transfected 293T: (B) and SK-N-SH (C) whole cell lysates.
Application
Western blot analysis of DJ-1 expression in HeLa whole cell lysate.
Application
Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic staining.
Application
Western blot analysis of DJ-1 expression in BT-20 (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of DJ-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
