Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic and extracellular staining.Application
Western blot analysis of human recombinant IL-15.Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic and membrane localization.| Product Name | IL-15 Antibody (H-114) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 49-162 representing mature IL-15 of human origin |
| Modification Notes | Interleukin-15 (IL-15), also designated IL-T, is a cloned cytokine which shares several biological activities but no sequence homology with IL-2. Human, mouse and simian IL-15 cDNA clones have been isolated and characterized. All three species encode a 162 amino acid residue precursor protein containing a 48 amino acid leader that is cleaved to generate the mature form of IL-15. IL-15 stimulates the proliferation of T cells and NK cells, while enhancing B cell expansion and antibody production. Unlike IL-2, IL-15 is not produced by lymphocytes, but appears to be produced by macrophages, epithelial lines, muscle and placenta. IL-15 has also been shown to be a chemoattractant for human blood T lymphocytes and to be able to induce lymphokine-activated killer (LAK) activity in NK cells as well as to be able to induce the generation of cytolytic effector cells. Studies have shown that IL-15 is the only other cytokine that shares the ∫ signaling subunit of the IL-2R. Evidence also suggests that like IL-2, IL-4 and IL-7, IL-15 utilizes the common IL-2R© subunit. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IL-15 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 49-162 representing mature IL-15 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 49-162 representing mature IL-15 of human origin |
| Entrez Gene | 3600 |
|---|
Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic and extracellular staining.
Application
Western blot analysis of human recombinant IL-15.
Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic and membrane localization.| Application Notes | recommended for detection of IL-15 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
