Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear staining of pneumocytes.Application
Western blot analysis of TTF-1 expression in non-transfected: (A) and human TTF-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of TTF-1 expression in TT (A), A549 (B) and SHP-77 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed TT cells showing nuclear localization.| Product Name | TTF-1 Antibody (H-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-190 mapping at the N-terminus of TTF-1 of human origin |
| Modification Notes | TTF-1 (thyroid transcription factor-1, BCH, BHC, NK-2, Nkx2.1, Nkx2A, TEBP, TTF1) is a member of the Nkx2 family of homeodomain-containing transcription factors and regulates the transcriptional activity of thyroid-specific genes. TTF-1 influences organogenisis and the maintenance of the differentiated phenotypes of various tissues including thyroid, lung and brain. TTF-1, which is present in the epithelium of the lung, regulates transcription of the surfactant proteins (SP) A, B and C and is essential for lung morphogenesis. In the thyroid, TTF-1 elevates the expression of thyroid specific markers, thyroglobulin, thyroperoxidase and thyrotropin receptors. TTF-1 interacts with SRC-1 and CBP in vitro. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of TTF-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-190 mapping at the N-terminus of TTF-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-190 mapping at the N-terminus of TTF-1 of human origin |
| Entrez Gene | 7080 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear staining of pneumocytes.
Application
Western blot analysis of TTF-1 expression in non-transfected: (A) and human TTF-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of TTF-1 expression in TT (A), A549 (B) and SHP-77 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed TT cells showing nuclear localization.| Application Notes | recommended for detection of TTF-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
