Application
Western blot analysis of CD84 expression in J774.A1 (A), RAW 264.7 (B), mouse PBL (C), IB4 (D), BW5147 (E) and FOX-NY (F) whole cell lysates.| Product Name | CD84 Antibody (M-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 200-329 mapping at the C-terminus of CD84 of mouse origin |
| Modification Notes | The human CD84 gene maps to chromosome 1q24 and is composed of at least eight exons, with an exon coding for the 5' UTR and the leader peptide, two exons coding for each of the two extracellular Ig-like domains, an exon encoding the hydrophobic transmembrane region and four exons coding for the cytoplasmic domains. The extracellular Ig-like domains share structural and sequence homology with a group of members of the Ig superfamily that include CD2, CD48, CD58 and Ly9. Five CD84 isoforms have been characterized, including CD84a, CD84b, CD84c, CD84d and CD84e, which are preferentially expressed on B lymphocytes, monocytes and platelets, where they act as their own ligand and are therefore costimulatory molecules. The CD84 isoforms are generated by alternative exon enhancement, reading frame shift and use of cryptic splice sites. The differential expression of potential sites of phosphorylation on the different isoforms may be a way to regulate CD84 activity in signal transduction. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD84 of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 200-329 mapping at the C-terminus of CD84 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 200-329 mapping at the C-terminus of CD84 of mouse origin |
| Entrez Gene | 12523 |
|---|
Application
Western blot analysis of CD84 expression in J774.A1 (A), RAW 264.7 (B), mouse PBL (C), IB4 (D), BW5147 (E) and FOX-NY (F) whole cell lysates.| Application Notes | recommended for detection of CD84 of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
