Application
Western blot analysis of Nup98 expression in non-transfected 293T: (A), human Nup98 transfected 293T: (B) and K-562 (C) whole cell lysates.| Product Name | Nup98 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 581-880 mapping within an internal region of nucleoporin Nup98 of human origin |
| Modification Notes | Nuclear pore complexes (NPCs) are the channels for the bi-directional movement of macromolecules between the nucleus and cytoplasm, and contain more than 100 different subunits. Many of them belong to a family called nucleoporins, which are characterized by the presence of O-linked N-acetylglucosamine moieties and a distinctive pentapeptide repeat (XFXFG). The NUP98 gene encodes precursor proteins that generate two nucleoplasmically oriented nucleoporins, Nup98 and Nup96. The O-linked glycoprotein, Nup98 is a component of the nuclear pore complex. Nup98 is essential for gastrulation, a developmental stage that is associated with rapid cell proliferation, but dispensable for basal cell growth. Nup98 plays a role in RNA export from the nucleus and it appears to be an essential component of multiple RNA export pathways. Nup98 is a member of the GLFG nucleoporin family. The t(7;11)(p15;p15) translocation in acute myeloid leukaemia fuses the genes for Nup98 and class I homeoprotein HoxA9. Nup98-HoxA9 fusion protein may promote leukemogenesis through inhibiting of HoxA9-mediated terminal differentiation and/or aberrant nucleocytoplasmic transport. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of nucleoporin Nup98 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 581-880 mapping within an internal region of nucleoporin Nup98 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 581-880 mapping within an internal region of nucleoporin Nup98 of human origin |
| Entrez Gene | 4928 |
|---|
Application
Western blot analysis of Nup98 expression in non-transfected 293T: (A), human Nup98 transfected 293T: (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of nucleoporin Nup98 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
