Application
Western blot analysis of Synapsin III expression in non-transfected: (A) and human Synapsin III transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of Synapsin III expression in mouse brain tissue extract.| Product Name | Synapsin III Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 31-120 mapping near the N-terminus of Synapsin IIIa of human origin |
| Modification Notes | Synapsins are synaptic vesicle-associated phosphoproteins that regulate synaptic vesicle exocytosis and may be involved in synaptogenesis. Evidence suggests that Synapsin I, Synapsin II and Synapsin IIIa are ATP-binding proteins that are regulated by Ca2+ and calmodulin binding. Ca2+ has been shown to stimulate ATP binding to Synapsin I, to have no effect on Synapsin II and to inhibit Synapsin III. Synapsin I and Synapsin II both undergo alternative splicing to produce two forms of each protein, Synapsin la and lb and Synapsin IIa and IIb, respectively. Synapsin III gives rise to at least three isoforms: Synapsin IIIa, IIIb and IIIc. Synapsin III plays unique roles both in early axon outgrowth and in the regulation of synaptic vesicle trafficking. In cultured mouse hippocampal neurons, synapsin III is expressed early during development, with levels peaking seven days after plating and declining thereafter. Synapsin III is highly concentrated in growth cones. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Synapsin IIIa, b and c of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 31-120 mapping near the N-terminus of Synapsin IIIa of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 31-120 mapping near the N-terminus of Synapsin IIIa of human origin |
| Entrez Gene | 8224 |
|---|
Application
Western blot analysis of Synapsin III expression in non-transfected: (A) and human Synapsin III transfected: (B) 293T whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of Synapsin III expression in mouse brain tissue extract.| Application Notes | recommended for detection of Synapsin IIIa, b and c of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
