Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing membrane and cytoplasmic staining of glandular cells.Application
Western blot analysis of TGFβ RI expression in human platelet whole cell lysate.Application
Western blot analysis of human recombinant TGF RI fusion protein.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Product Name | TGFβ RI Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 26-125 mapping within the extracellular domain of TGFβ RI of human origin |
| Modification Notes | A total of three members of the TGF∫ family, namely TGF∫1, TGF∫2 and TGF∫3, have been identified in mammals. Each is synthesized as a latent precursor that is subsequently cleaved forming the 112 amino acid growth factor which becomes active upon dimerization. TGF∫s mediate their activity by high affinity binding to the TGF∫ receptor type-II (TGF∫ RII) with a cytoplasmic serine-threonine kinase domain. For signaling growth inhibition and early gene responses, TGF∫ RII requires both its kinase activity and its association with a TGF∫-binding protein, designated TGF∫ receptor type-1 (TGF∫ RI). TGF∫ RI is a 503 amino acid single-pass type I membrane protein that is expressed ubiquitously and, with TGF∫ RII, functions as a receptor for TGF∫. Defects in the gene encoding TGF∫ RI are the cause of aortic aneurysm familial thoracic type 5 (AAT5), Loeys-Dietz syndrome type 2A (LDS2A) and Loeys-Dietz syndrome type 1A (LDS1A). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TGFβ RI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 26-125 mapping within the extracellular domain of TGF&beta RI of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 26-125 mapping within the extracellular domain of TGFβ RI of human origin |
| Entrez Gene | 7046 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing membrane and cytoplasmic staining of glandular cells.
Application
Western blot analysis of TGFβ RI expression in human platelet whole cell lysate.
Application
Western blot analysis of human recombinant TGF RI fusion protein.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Application Notes | recommended for detection of TGFβ RI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
