Application
Western blot analysis of AChRα5 expression in mouse brain tissue extract.| Product Name | AChRα5 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 370-419 mapping within a cytoplasmic domain of AChRα5 of human origin |
| Modification Notes | Members of the ligand-gated ion channel receptor family are characterized by their fast transmitting response to neurotransmitters. Two important members of this family are the nicotinic acetylcholine and glutamate receptors, both of which are composed of five homologous subunits forming a transmembrane aqueous pore. These transmembrane receptors change conformation in response to their cognate neurotransmitter. Nicotinic acetylcholine receptors (AChRs) are found at the postsynaptic membrane of the neuromuscular junction and bind acetylcholine molecules, allowing ions to move through the pore. Glutamate receptors are found in the postsynaptic membrane of cells in the central nervous system. The activity that is generated at the synapse by the binding of acetylcholine is terminated by acetylcholinesterase, an enzyme that rapidly hydrolyzes acetylcholine. AChRå5, also known as LNCR2 or CHRNA5 (cholinergic receptor, nicotinic, alpha 5), is a 468 amino acid multi-pass membrane protein belonging to the ligand-gated ionic channel family and is involved in the mediation of fast signal transmission at synapses. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AChRα5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 370-419 mapping within a cytoplasmic domain of AChR&alpha 5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 370-419 mapping within a cytoplasmic domain of AChRα5 of human origin |
| Entrez Gene | 1138 |
|---|
Application
Western blot analysis of AChRα5 expression in mouse brain tissue extract.| Application Notes | recommended for detection of AChRα5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
