Application
Western blot analysis of CREB-2 expression in K-562 whole cell lysate.Application
Western blot analysis of CREB-2 expression in non-transfected: (A) and human CREB-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CREB-2 expression in non-transfected 293T: (A), human CREB-2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of CREB-2 expression in HeLa nuclear extract (A) and A-431 (B), K-562 (C) and Jurkat (D) whole cell lysates.| Product Name | CREB-2 Antibody (H-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-290 of CREB-2 of human origin |
| Modification Notes | Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promoter and enhancer elements. The cAMP response element (CRE), one of the best studied of such elements, consists of the palindromic octanucleotide TGACGTCA (1,2). Several CRE binding proteins have been identified within the ATF/CREB family, the best characterized of which include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3 (3–6). These proteins share highly related COOH terminal leucine zipper dimerization and basic DNA binding domains but are highly divergent in their amino terminal domains (5,6). Although each of the ATF/CREB proteins appear capable of binding CRE in its homodimeric form, certain of these also bind as heterodimers, both within the ATF/CREB family and even with members of the AP-1 transcription factor family (7) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of CREB-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-290 of CREB-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-290 of CREB-2 of human origin |
| Entrez Gene | 468 |
|---|
Application
Western blot analysis of CREB-2 expression in K-562 whole cell lysate.
Application
Western blot analysis of CREB-2 expression in non-transfected: (A) and human CREB-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CREB-2 expression in non-transfected 293T: (A), human CREB-2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of CREB-2 expression in HeLa nuclear extract (A) and A-431 (B), K-562 (C) and Jurkat (D) whole cell lysates.| Application Notes | recommended for detection of CREB-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
